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In order to study the effects of seed priming with 24-epibrassinolide (EBL) (0, 0.1, 1 mg/l) on the increase of the tolerance of tomato plants (Lycopersicon esculentum Mill.) to salinity (0, 70 and 140 mM NaCl), factorial experiments were conducted in a completely randomized design under greenhouse conditions. Results showed that salt stress significantly decreased the growth of tomato plants. However, priming with EBL (1 mg/l) significantly decreased the negative effects of NaCl (particularly at 140 mM). As a result, EBL increased the fresh and dry weights (<2 folds), total chlorophyll (+72%), carotenoids (+ 2 folds), free proline (+3 folds), electrolyte leakage (-18.6%), Na/K in the roots (-57.5%) and shoots (-62%) compared with salinity control. It could be concluded that priming with epibrassinolide resulted in enhanced salt tolerance in tomato plants via increment in free proline, photosynthetic pigments and decrease in electrolyte leakage and Na/K ratio in the tissues.

Effects of seed pretreatment with 24-brassinolide on physiological and biochemical characters in tomato plants under salt stress