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Bacterial spot, caused by Gram-negative phytpathogenic bacteria Xanthomonas campestris pv. vesicatoria is one of the de vastating diseases, that causes significant decrease in tomato and peper yield and economic loss. With the aim of search of natural means against the diseases study has been conducted on the influence of immune stimulation preparation of plant origin Lignohumate AM on tomato protection from bacterial spot while seeds inoculation with disease causative pathogen X. vesicatoria. Inoculation of tomato seeds have been carried out with X. vesicatoria strains No2255 and No2256, isolated by us from bacterial spot diseased tomato leaves, collected in Kartli Region, Georgia in 2014-2015 years, as well as with Ref. strain NCPPB422. For the seeds inoculation 24h grown on 2% potato agar culture (108 CFU/ml) washout suspensions were used. In some variants 4hour water soaked seeds were simultaneously inoculated by X. vesicatoria different strains and treated with lignohumate during 1 hour at 24oC. Control variant – intact seeds were soaked in sterile water during 5 hours at 24oC. Some seeds before or simulatneously with bacteria inoculation were treated with 10% Lignohumate AM solution. As a result of inoculation with X. vesicatoria virulent strains the amount of grown seedlings decreased by 75% as compared to intact seeds. Treatment of seeds with Lignohumate, simultaneously or before the inoculation by X. vesicatoria, resulted in 3.0-3.5 times increase in growth and development as compared to untreated inoculated seeds. The results indicate on efficiency of lignohumate AM in protection of tomato seeds from X. vesicatoria infection.

Influence of Lignohumate on tomato seeds germination and growth under inoculation with bacterial spot causative pathogen Xanthomonas vesicatoria