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The study aims to investigation the presence of Bovine Parainfluenza-3 Virus (BPI-3V) by using direct Enzyme Linked Immunosorbent Assay (ELISA) and Real Timequantitative Polymerase Chain Reaction RT-qPCR technique and evaluation some clinical and epidemiological features of the disease. One hundred forty seven (147) animals of different age (6 months to 8 years) and sex from different regions of Al-Diwaniya governorate that showed respiratory signs were examined between November 2012 and April 2013. Results of clinical study showed that there was increase in body temperature up to 40 C 0 , serous watery nasal discharge, increase respiratory rate, abnormal breath sound (loud, harsh sound, whistling or wheezing), and coughing. The infection rate by using direct ELISA test was (30.26 %). The spreading rate of BPIV-3 in relation to ages, regions and months of the years was (48%) in age group 6 months-3 years, highest rate (60%) in December as compare with other months and Sedeer region recorded infection rate (40%). The results of Real Time-qPCR showed high infection rate of BPI-3 virus 55.13% in cattle population as high sensitivity of this technique. Higher percentage recorded in tracheal tissue sample 60.60 % as compare with lungs tissue and nasal swabs in percentage 54.54% and 50%, respectively. In conclusion there was a characteristic epidemiological feature of spreading of BPIV-3 in depending on age groups, different regions, and different months of the year.

Seroprevalence and molecular detection of Bovine Parainfluenza-3 Virus (BPI-3V)