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El objetivo de la presente investigacion fue hidrolizar harina de plumas de pollo para la obtencion de queratina. El diseno metodologico de la investigacion fue experimental; se realizaron cuatro fermentaciones correspondientes a un diseno experimental completamente al azar, resultantes de considerar factores [concentracion de sustrato (18 g/L y 23 g/L) y concentracion del inoculo (2 y 3 g/L), dos niveles para cada factor]. La determinacion de la concentracion de nitrogeno total se efectuo mediante espectrofotometria UV-Visibles, el metodo utilizado fue la digestion de persulfato de potasio (K2S2O8), la concentracion maxima de nitrogeno se alcanzo en el tratamiento 2 (1,5 %), lo cual multiplicado por el factor proteico 6,25 dio un porcentaje de proteina de 9,4% de queratina en el hidrolizado, presentando diferencia significativa (p<0,05) del resto de tratamientos, al cabo de 24 horas de retencion hidraulica. En conclusion, es posible la hidrolisis enzimatica de harina de plumas de pollo utilizando el Bacillus subtilis como productor de proteasas.  ABSTRACT The objective of this research was hydrolyzed feather meal of chicken for the production of keratin. The methodological research design was experimental; four fermentations, corresponding to a completely randomized experimental design, resulting from considering factors [substrate concentration (18 g / L and 23 g / L) and concentration of inoculum (2 and 3 g / L), two levels was performed for each factor]. Determining the total nitrogen concentration was performed by UV-Visible spectrophotometry, the method used is the persulfate potassium digestion (K2S2O8), the maximum concentration of nitrogen is reached in the treatment 2 (1.5%) which multiplied by the factor protein 6.25 gives a percentage of 9.4% protein in the hydrolyzed keratin, showing significant difference (p<0.05) from other treatments at 24 hours hydraulic retention. In conclusion the enzymatic hydrolysis of chicken feather meal is possible using the Bacillus subtilis as a producer of proteases.

ciencia unemi/ciencia unemi (en línea)

Queratina a partir de la hidrólisis enzimática de harina de plumas de pollo, utilizando queratinasas producidas por Bacillus subtilis / Getting keratin from feather meal using keratinases produced by Bacillus subtilis