English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

This work demonstrates sparse cell detection in mL samples, using magnetic bead manipulation on an ElectrowettingOn-Dielectric (EWD) chip. Sparse sample detection was performed in two steps: cell capture off chip from the starting solution with a microelectromagnet and on-chip fluorescent signal detection on an EWD chip. In the first step, immunological reactions enable the binding between target cells and antibody-coated magnetic beads, which enabled sample capture with high cell survival rates. In the second step, fluorescent detection is achieved on an EWD chip via fluorescent signal measurement and two-dimensional magnetic bead concentration. Magnetic bead concentration is controlled with an integrated microelectromagnet, a planar set of half-circle-shaped current-carrying wires embedded in an actuation electrode of an EWD device. This two-dimensional wire structure serves as a microelectromagnet capable of segregating magnetic beads into an area on the order of 10 μm2 with a resulting improvement in Signal-To-Noise Ratio (SNR) of 30 times. Simple device integration ensures that the magnetic bead manipulation and the EWD function can be operated simultaneously without introducing additional steps in the EWD chip fabrication process. Immunological reaction kits were selected in order to ensure the compatibility of target cells, magnetic beads and EWD functions. The magnetic bead choice ensures the binding efficiency and survivability of target cells. The magnetic bead selection and binding mechanism used in this work can be applied to a wide variety of samples with a simple switch of the type of antibody. Sparse cell fluorescent measurements with good SNR are made possible by using fluorescent stains and a method of concentrating cells attached to beads into a small detection area. Theoretical limitation of the entire sparse sample detection system is as low as 1 colony forming unit/mL (CFU/mL). DOI: 10.29011/ 2577-2260. 100045

Cell Manipulation and Detection Enhancement Enabled by a Microelectromagnet Integrated with a Digital Microfluidic Device