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Biochemical analysis in horses is an important aid for determining correct clinical diagnosis of general, infectious, and some parasitic diseases. This work studied the biochemical and mineral indices in mares of two breeds: the Norik breed Muraň Plain type and the Hucul breed. A total of 34 mares of the Norik breed Muraň Plain type (aged 15.18 ± 5.99 years) and 28 Hucul mares (aged 9.03 ± 5.50 years) were used. Blood serum was analysed using the biochemical analyser Cobas c111 (Roche, Switzerland). Significant difference (P < 0.05) was found between the Norik breed Muraň Plain type and the Hucul mares in aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase and gamma-glutamyltransferase activity; significant difference (P < 0.01) was found in urea values; and highly significant difference (P < 0.001) was found in glucose values. The mineral profile elements showed a highly significant differences (P < 0.001) between the Norik breed Muraň Plain type and the Hucul mares in phosphorus, magnesium, iron, chloride, potassium, and sodium concentrations. The results confirmed that there are significant differences between horse breeds in some biochemical indices. Therefore, it is appropriate to determine reference values for other horse breeds, as well. To our knowledge, this is the first report that compares biochemical and mineral indices between the Norik breed Muraň Plain type and the Hucul breed. Cold-blooded horse, haematological indicators, selected metabolic indicators The reference values of indices determined in blood may vary according to breed and are affected by age and to a certain extent also by breeding conditions (Bauer et al. 1984, 1985). Appropriate interpretation of the results of the mineral and biochemical profile requires specific reference values for each animal species (Doubek et al. 2003). Differences between thoroughbreds, standardbreds and hot-blooded horses have already been published (Parry 2003). It is now necessary to acquire reference values for different horse breeds. The Norik breed Muraň Plain type is one of the cold-blooded horse breeds mainly intended for work in forestry and agriculture. It is frequently used for hippotherapy and recreational riding for its good character, excellent manoeuvrability, and constitution. In recent history of horses working in forestry the Norik breed Muraň Plain type has gained a leading position among other cold-blooded breeds and has steadily shown quality performances in competitions. An important aspect of the Norik breed Muraň Plain type is also its contribution to the rural landscape and heritage conservation of Slovakia (Duruttya and Pernica 2000). The Hucul is a typical small mountain horse coming from the Carpathian Arc and a representative of the autochthonous horse breed in the Carpathian Basin countries. It belongs to the cultural biological heritage of the Carpathian Basin countries. Hucul horses

Comparison of some biochemical and mineral indices among Norik breed Muráň Plain type and Hucul breed mares