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Ketoprofen residues have been found in surface water where they present a potential risk to nontarget aquatic species. The aim of this study was to assess the impact of ketoprofen in surface waters on fish under experimental conditions. Subchronic toxic effects on 300 embryos and larvae of common carp (Cyprinus carpio) were investigated during a 30 day toxicity test with concentrations of ketoprofen: 0.003, 2.1, 6.3 and 21 mg/l. The exposure to ketoprofen showed no effect on mortality, but we observed significant delay (P < 0.05) in hatching in day 3 in fish exposed to all concentrations of ketoprofen. Significant delays (P < 0.05) in development were revealed at 2.1, 6.3 and 21 mg/l ketoprofen. On the basis of weight and growth rate evaluation and the determination of developmental stages, the lowest observed effect concentration was 0.003 mg/l. According to these results, the reported environmental concentration of ketoprofen in Czech rivers could have a negative effect on the growth and development of carp embryos and larvae. These tests have not yet been performed. Further research and search for a mechanism to reduce the incidence of ketoprofen in the waters by better wastewater treatment is required.

The effects of subchronic exposure to ketoprofen on early developmental stages of common carp