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The objective of four trials performed on specific-pathogen-free and commercial chickens, either of light or heavy hybrids, was to evaluate the new vaccine delivery method to newly hatched chickens using commercial La Sota vaccine. The vaccine was given by means of nebulization using an ultrasonic device producing homologous aerosol of particles ranging 3-5 microns in diameter. Chickens were exposed to the La Sota vaccine for 30, 60 or 300 s in a closed chamber of the device, thus enabling constant particle size during vaccination. No adverse reaction to the given vaccine was recorded, and the immunity, developed no later than 7 days after vaccination, lasted for at least 49 days which was confirmed by challenge infection using Herts 33 strain of Newcastle disease virus. Maternal antibodies did not influence the development of immunity. Regarding the mode of vaccination, the described method is suitable for the control of Newcastle disease in both big poultry enterprises as well as small backyard flocks when newly hatched chickens are supplied from local hatcheries. Ultrasonic nebulizer, chicks, vaccination, La Sota strain, immune response, Newcastle disease Newcastle disease (ND) still remains a serious poultry health problem, causing mortality and therefore economic loss. Appearance of virulent ND viruses (vNDV)

Immunogenicity and Safety of La Sota Strain of Newcastle Disease Virus Administered to Newly Hatched Chicks by Nebulization