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Velí‰ek J., Z. Svobodová: Anaesthesia of Common Carp (Cyprinus carpio L.) with 2-phenoxyethanol: Acute Toxicity and Effects on Biochemical Blood Profile. Acta Vet. Brno 2004, 73: 247-252. The aim of the present study was to assess acute toxicity of the 2-phenoxyethanol anaesthetic in carp, and assess changes in their tissues using the biochemical blood profiles. Acute toxicity values of 2-phenoxyethanol in the carp fry determined by the OECD 203 method "Acute toxicity test in fish" were 10minLC50 0.39; 10minLC0.1 0.30; 10minLC99.9 0.50 ml⋅l-1, 96hLC50 0.17; 96hLC0.1 0.13; and 96hLC99.9 0.26 ml⋅l-1. A biochemical analysis of blood plasma (VETTEST 8008 analyser, Medisoft Co.) was used to determine changes in the biochemical profile of blood in carp stockfish due to anaesthesia by 2-phenoxyethanol. The analyses were made immediately prior the anaesthesia and after a 10 min and 24 h exposure to the anaesthetic at a concentration of 0.30 ml⋅l-1. A total of 30 two-year-old carp were examined. A significant increase in blood plasma alanine aminotransferase (ALT) levels from 0.15 µkat⋅l-1 to 0.35 µkat⋅l-1 (P < 0.05) was found 24 h after anaesthesia. The other indices monitored (cortisol, glucose (GLU), total proteins (TP), triglycerides (TRIG), ammonia (NH3), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and creatine kinase (CK)) showed no significant differences among the groups of fish examined. The results showed that 2-phenoxyethanol at a concentration of 0.30 ml⋅l-1 is not completely safe for carp fry and two-year old carp. In view of different sensitivities in different age group mentioned above, the authors assume that concentration will be safe for broodstock carp. LC50, blood plasma, cortisol, glucose, proteins, enzymes