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Trávniãek M., D. Kováãová, M.R. Bhide, P. Zubrick˘, L. âisláková:Detection of IgG Antibodies against Chlamydophila abortus in Sheep with Reproductive Disorders. Acta Vet. Brno 2003, 72: 95-99. Chlamydophila (Chl.) abortus is the most important causative agent of the enzootic abortion and other chlamydial infections of sheep. Complement fixation (CF) test is a very useful serological method as screening test, but the diagnosis can be complicated by non-specific, false positive reactions with some Gram-negative bacteria as well as with Chl. pecorum. Diagnosis can be improved by simultaneous use of indirect (i)ELISA. Moderate agreement (Kappa - 0.568) between CF test and iELISA was observed in this study. We observed 36.0 % (75/208) positive samples from clinically healthy sheep and 63.2 % (91/144) in the sheep with reproductive disorders by detecting anti-Chl. abortus IgG antibodies using iELISA. When the same sera samples were investigated by CF test, only 13.9 % seropositivity was observed in clinically health sheep whereas in the group with reproductive disorders, positivity observed was 45.8%. Sensitivity and specificity of iELISA used in this study was found to be higher than CF test. Chlamydophila abortus, IgG antibodies, sheep, iELISA, CF test