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Vávrová M., H. Zlámalová Gargo‰ová, E. ·ucman: Impact of Environmental Pollution on Contamination of Locally Grown Roughage. Acta Vet. Brno 2002, 71: 389-400. Contamination of ration components and roughage fed to dairy cows and finishing bulls in two different agricultural ecosystems in the district of Uherské Hradi ‰tû (localities Buchlovice and Stupava) was monitored. Whereas the site Buchlovice was characterised by intensive agricultural production, the site Stupava was located in the protective zone of the Koryãany water reservoir and ecological agriculture was practised in a part of this area. Materials used for analyses included irrigation water, grass growth, haylage, wheat, roughage, preserved feeds and trough samples of feed. Samples of alfalfa and clover were also collected as ration components and bioindicators of plant origin. From each sample kind and/or sampling site 15 samples were collected. In addition to feeds, bovine milk and beef liver and muscle ( m. longissimus dorsi) samples were analysed (10 samples of animal material). Imissions were measured by the mobile laboratory of Regional Hygienic Services, Brno, allowing the determination of SO 2, NOx, CO, O3, fly ash. The samples were analysed for polychlorinated biphenyls (PCB) by high resolution gas chromatography with electron capture detection. Also tested for PCB residues were milk and tissue samples collected from dairy cows and bulls. The concentrations of indicator PCB congeners did not exceed the safety limit in any of the sampling areas (mean concentrations of PCB in feed between 1-2 µg·kg -1; mean concentration of PCB and animal tissues between 2-9 µg·kg -1). Calculation of the transfer coefficient Q proved to be a suitable tool for the assessment of residue transfer (dairy cows 0.2-1.0; bulls 0.3-1.0). Feed was identified as an important contamination source for animal tissues and milk. Polychlorinated biphenyls, agricultural ecosystem, transfer coefficient Q, food chains