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Sahin K., N. Sahin: Effects of Chromium Picolinate and Ascorbic Acid Dietary Supplementation on Nitrogen and Mineral Excretion of Laying Hens Reared in a Low Ambient Temperature (7 oC). Acta Vet. Brno 2002, 71: 183-189. The effects of chromium (chromium picolinate, CrPic) and ascorbic acid (L-ascorbic acid) supplementation on nitrogen (N), ash and mineral retention in laying hens (Hy-Line) reared under a low ambient temperature (7 oC) was evaluated. One hundred and twenty laying hens (32-week- old) were divided into four groups, 30 hens per group. The laying hens were fed either a basal diet or the basal diet supplemented with either 400 µg of Cr/kg of diet, 250 mg of L-ascorbic acid /kg of diet, or 400 µg of Cr plus 250 mg of L-ascorbic acid/kg of diet. Retention of N, ash, Ca, P, Zn, Fe and Cr were highest with the combination of chromium and ascorbic acid and were lowest with the control diet ( P< 0.05). Accordingly, excretion of N, ash Ca, P, Zn, Fe and Cr was lesser in chromium and ascorbic acid supplemented groups than the control ( P< 0.05), that of combination of supplemental chromium and ascorbic acid being lowest. Results of the present study show that supplementing ascorbic acid and chromium, particularly as a combination, improved retention of mineral and decreased excretion of nitrogen, ash, Ca, P, Zn, Fe, and Cr in laying hens. Such a combination of supplementation can offer a potential protective management practice in preventing the detrimental effects of cold stress in laying hens. The results of the present study also show that chromium and vitamin C had additive effects on parameters measured at the present study. Cold stress, chromium, ascorbic acid, mineral excretion, laying hen

acta veterinaria brno

Effects of Chromium Picolinate and Ascorbic Acid Dietary Supplementation on Nitrogen and Mineral Excretion of Laying Hens Reared in a Low Ambient Temperature (7 °C)