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Pavlata L., J. Illek, A. Pechová, M. Matûjíãek: Selenium Status of Cattle in the Czech Republic. Acta Vet. Brno 2002, 71: 3-8. Selenium status was assessed directly by determination of selenium concentration, or indirectly by measurement of glutathione peroxidase activity in whole blood samples collected from 879 cattle (733 cows, 63 calves, 42 heifers, 41 finishing bulls) reared on 93 farms in 12 of the 14 regions of the Czech Republic. Selenium deficiency or marginal values were found in 50 % of the tested animals and on 54 % of the farms. In terms of animal categories, deficient or marginal selenium status was found in 42 % of cows, 80 % of calves, 100 % of heifers, and 90 % of bulls. Selenium- deficient herds were detected in almost all regions of the Czech Republic. The lowest selenium concentrations (< 20 µg.l -1 whole blood) were found in western, northern, and north-eastern Bohemia and in northern Moravia. It is evident that selenium deficiency in cattle is a topical problem in the Czech Republic and that selenium status must be monitored within preventive diagnostics in all age groups of cattle to decide correctly on the most effective way of supplementation.