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research, we investigated the GSH-Px activity in a group of calves (n = 47) that had not received supplements added to the basal diet. The research was carried out in the north-west Croatia, in the region known to be poorer in selenium. We determined GSH-Px activity in whole blood by the "Ransel" method adapted to a Technicon RA-1000 at 37 °C. Determination of Se concentration was done with a modification of the method given by Perkin-Elmer HGGS. The mean value of the Se concentration in the whole blood of the first group of calves was 200.22 ± 45.2 µg.l-1 and pertinent average GSH-Px activity was 764.6 ± 197.8 µkat.l-1. From the results obtained, a correlation between Se and GSH-Px was calculated (r = 0.82; P < 0.001). The mean values of the GSH-Px activity in herd that did not receive a supplement were 435.3 ± 155.76 µkat.l-1. Out of 47 animals of the second herd, four animals (8.51%) had lower values than those recommended as sufficient. This study confirmed that after the calculation of the correlation between Se and GSH- Px, glutathione peroxidase activity determination can be used as a rapid and simple proxy for the determination of selenium concentration in whole blood.

Correlation Between Glutathione Peroxidase Activity and The Quantity of Selenium in The Whole Blood of Beef Calves