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A phenolic extract containing lipoplysaccharide and residual nucleic acids (BBE-LPS) was prepared from selected strains of Bordetella bronchiseptica. Toxicity and immunostimulatory activity of the free extract and the extract incorporated into large unilamellar (LUV) and multilamellar (MLV) liposomes were tested in laboratory animals. The free extract significantly enhanced the resistance of mice to infections with P. multocida and pseudorabies virus at doses down to 1 mug per animal. Incorporation into liposomes slightly reduced the toxicity of the extract. The incorporated extract enhanced the resistance to infection in mice, but its activity did not exceed that of the free extract.

Nonspecific Stimulation or Resistance of Mice to Infection by Lipopolysaccharide of Bordetella bronchiseptica Incorporated into Liposomes