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The detection of Early Pregnancy Factor (EPF) is not feasible in some species that have only limited ability to form spontaneous E rosettes. Since EPF is not species specific, a suitable model for its detection in sera of these species based on the capacity of some other species to form active spontaneous E rosettes is arched. We have investigated the influence of incubating pig lymphocytes in sera of mice, rabbits, dogs, sheep, horses, cows, women and sows (control) upon the percentage of active E rosette forming cells (ERFCs). The incubation of pig lymphocytes in xenogeneic sera was not followed by any significant alterations in the percentage of ERFCs, but this parameter was changed significantly (27.15 +/- 1.31 versus 39.52 +/+/4. 78%, p < 0.01) by increasing the lymphocytes/sheep red blood cells ratio from 1 : 10 to 1 : 30.

The Percentage of Active E rosette Forming Cells After Incubation of Pig Lymphocytes in Xenogeneic Sera