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Tissue samples obtained from the pyloric part (antrum pyloricum) of the stomach of 6 mice and 4 adult cats were fixed in Bouin's solution, embedded in paraplast and sectioned at 5 to 7-mu-m. Endocrine G-Cells were demonstrated in the sections immunohistochemically using a MILAB-ICC kit for immunohistochemical demonstration of gastrin. The final colour visualization was achieved by the binding of the PAP complex. The study of the distribution, cytomorphology and G-cell types in the epithelium of the mucosa and glands yielded the following results: 1. Endocrine G-cells were scattered diffusely in the basal part of the mucosa. 2. In the cat they occupied the lower half of the height of the mucosal epithelium, in the mouse they were bound topographically to the basal quarter or fifth of the total height of the mucosal epithelium. 3. In both species the surface cells were immunopositive; positive reactions were observed locally also in non-cellular structures of the mucosal surface. 4. In both species two types (open type and closed type) of endocrine G-cells were found to occur. 5. In both cats and mice open-type G-cells were fewer in number than closed-type cells. 6. Cells with polymorphic cytoplasmic projections were observed in both species, but more frequently in the mouse than in the cat.

Immunohistochemical Localization of Endocrine G-Cells in the Epithelium of the Pars pylorica Mucosa of the Cat and Mouse Stomach