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Svobodova Vlasta, M. Nevole: Use o/the Muscle Digestion Method and Indirect Immunofluorescence Reaction in the Diagnosis 0/ Sarcocystom in Sheep. Acta vet. Bmo, 59,1990: 157-170. A total of 1 014 sheep (522 ewes, 150 rams and 342 lambs) from the catchment­ -areas of the abattoirs of TiSnov, Klatovy, Strakonice and Nymburk were subjected to direct and indirect examination for sarcocystosis between October 1985 and June 1988. Direct examination with the muscle digestion method revealed muscle cysts in 75.10 %,64.67 % and 35.67 % of the ewes, rams and lambs, respectively. The ratio of macrocysts to microcysts was 22 : 608. Macrocysts were found only in adult sheep (in 3.27 % of the ewes and rams) and, except one case where they were de­ tected in both the the oesophagus and the diaphragm, were located exclusively in the oesophagus. Microcysts were located predominantly in the diaphragm and the intensity of their occurrence increased significantly (P < 0.005) with the age of the animals. Serological examination of the blood using the indirect immunofluorescence reaction (IFR) detected specific antibodies in 88.70 %, 91.22 % and 94.44 % of the ewes, rams and lambs, respectively. In adult sheep the results of the two me­ thods of examination coincided in 71.87 % ofthe animals, whereas a high dynamic discrepancy was found in 61.69 % of the lambs. A highly significant (P < 0.005) correlation was found between the direct demonstration of muscle cysts and the specific antibody titre of 40 and higher as recorded for the IFR.

Use of the Muscle Digestion Method and Indirect Immunofluorescence Reaction in the Diagnosis of Sarcocystosis in Sheep