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Koudela B.: Activity of Aldolase in the Course of Experimental Trichinellosis in Guinea-pigs. Acta vet. Brno, 52, 1983; 169 -176. The activity of aldolase was determined in blood plasma and homogenates from the duodenal wall, masseter and heart musculature at different intervals after experimental infection of guinea pigs by Trichinella spiralis larvae. There were significant changes of enzymatic activity in all tissues studied. The dynamics of changes in the course of experimental trichinellosis was closely related to the life cycle of the parasite. The changes were due to altered permeability of cell membranes as well as to mechanical affection of surrounding tissue. Intracellular enzyme, intravital diagnosis, blood plasma, tissue homogenates. Trichinellosis is generally associated with changes in activity of enzymes; in the blood serum it is increased whereas the enzyme activity in skeletal muscles is simultaneously decreased. The peak of the changes lays in the 4th and 5th week post infection. They are due to enhanced permeability of cell membranes and involve above all cytoplasmic enzymes of low molecular w~ight. Localisation in the cytoplasm and low molecular weight facilitate the release of enzymes into extracellular space and blood. In the following phase of trichinellosis, the synthesis of muscular enzymes is increased and the permeability of cellular membranes is stabilized within physiological limits (Poznanska 1975). The changes in enzyme activity are not specific for trichinellosis, they occur with different myopathies as well. Papers concerning enzymes of host infected by T. spiralis are mostly casuistic in character and focused on the muscular phase of trichinellosis (Boczon et al. 1969, Kassur et al. 1969, Malik et al. 1960, Poznanska 1975, Wisniewska 1969). Our present ttial was based on the assumption that changes in aldolase activity are to be expected not only in the muscular but also in the intestinal phase of trichinellosis. Material and Methods Aldolase activity determination of physiological values The activity of plasma aldolase was measured in 22 guinea-pigs (females, 470-670 g). Effects of age, training and food composition on enzymatic activity (Laudahn 1963) were eliminated by selection of experimental animals and husbandry conditions. Blood samples were collected by heart puncture and centrifuged in heparinised tubes at 3.000 r. p. m. for 10 minutes. After blood collecting the guinea-pigs were killed in ether and samples were collected from heart muscles, left masseter and duodenal wall. The tissue samples were thoroughly washed in 0.1 M phosphate buffer and fat and connective tissue were removed. After drying by filtration paper, 200 mg of tissue were cut to pieces of approximately 1 mm. The so prepared tissue was immersed in 10 ml of 0.1 M phosphate buffer and homogenized in a cooled pestle homogenizator for 2 minutes. The centrifugation of homogenates was carried out in a cooled centrifuge at 5.000 r. p. m. for 10