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Meat Inspection Center of Saitama Prefecture Summary The cause, the pathogenic mechanism, the preventive and the inspection method of food poisoning associated with raw horse meat are described here. From epidemiological analysis of symptomatic complaint cases, raw horse meat turned out to be cause food. A lot of Sarcocystis cysts were observed in horse meat by parasitological examination. Sarcocystis parasitic in horse meat was identified as S. fayeri from the morphological and biological characteristics. The intestinal loop test indicated that the cyst and the bradyzoites of S. fayeri in raw horse meat revealed the enterotoxicity, which was due to 15 kDa protein contained in the bradyzoite. The enterotoxicity of sarcocysts was abolished by freezing for 18 hours or more at -22°C. The presence of sarcocysts in horse meat can be confirmed by conventional microscopic observation and by the analysis of 18SrDNA gene sequence using PCR method.

Food Poisoning Caused by Sarcocystis fayeri Associated with Ingestion of Raw Horse Meat