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We report, for the first time, a multi-confocal Fluorescence CorrelationSpectroscopy (mFCS) technique which allows parallel measurements at differentlocations, by combining a Spatial Light Modulator (SLM), with an ElectronMultiplying-CCD camera (EM-CCD). The SLM is used to produce a series of laserspots, while the pixels of the EM-CCD play the roles of virtual pinholes. Thephase map addressed to the SLM is calculated by using the spherical waveapproximation and makes it possible to produce several diffraction limitedlaser spots, either aligned or spread over the field of view. To attain fastenough imaging rates, the camera has been used in different acquisition modes,the fastest of which leads to a time resolution of 100 $\mu$s. We qualified theexperimental set-up by using solutions of sulforhodamine G in glycerol anddemonstrated that the observation volumes are similar to that of a standardconfocal set-up. To demonstrate that our mFCS method is suitable forintracellular studies, experiments have been conducted on two stable celllines: mouse embryonic fibroblasts expressing eGFP-actin and H1299 cellsexpressing the heat shock factor fusion protein HSF1-eGFP. In the first case wecould recover, by analyzing the auto-correlation curves, the diffusion constantof G-actin within the cytoplasm, although we were also sensitive to the complexnetwork of interactions with F-actin. Concerning HSF1, we could clearly observethe modifications of the number of molecules and of the HSF1 dynamics duringheat shock.

Multi-confocal Fluorescence Correlation Spectroscopy : experimental demonstration and potential applications for living cell measurements