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This review presents comparative results of simultaneously conducted studies on proteins responsible for the haemadsorption and serotype-specific properties of African swine fever virus (ASFV). An ASFV gene EP402R (or LMW8-DR) encoding protein CD2v homologous to murine, human or porcine T-cell adhesive receptor was found. The CD2v was shown to be directly involved into a haemadsorption process, and expressed in ASFV-infected cells as a glycoprotein with a molecular weight of approximately 105-110 kDa. In the presence of a glycosylation inhibitor, tunicamycin, its molecular weight is about 42 kDa. In ASFV-infected cells labeled with 3 H-glucosamine or 14 C-sodium acetate, a virus-specific major glycoprotein with a molecular weight of 110-140 kDa (gp 110-140) was identified using radioimmunoprecipitation assay. Using ASFV reference strains belonging to seroimmunotypes I-IV and the corresponding antisera active in haemadsorption inhibition assay (HADIA), we determined that gp 110-140 defines the serotype specificity. Genotyping on the basis of the genetic locus encoding the CD2v and a C-type lectin protein also showed a concurrence with the grouping of ASFV isolates and strains based on their seroimmunotypes. Immunization of pigs with the gp 110-140 within liposomes, or a recombinant haemagglutinin (CD2v) protected 67 to 100% of animals from death due to their subsequent infection with homologous virulent ASFV strains. Based on the physico-chemical and biological characteristics of the gp 110-140 and CD2v it is suggested that they are one and the same virus-specific glycoprotein crucial for induction of the immunological protection against ASF. Key words: ASFV; seroimmunotypes; serotype; glycoproteins; gp 110-140; CD2v; protectivity SEROTIPNO-SPECIFICEN IN HEMADSORPCIJSKI PROTEIN VIRUSA AFRISKE PRASICJE KUGE A.D Povzetek: Pregledni clanek predstavlja primerjavo rezultatov socasno izvedenih raziskav o beljakovinah, ki so pomembne za hemadsorpcijo in serotipno specificne lastnosti virusa afriske prasicje kuge (ASFV; iz angl. african swine fever virus). Pri virusu ASFV je bil odkrit gen EP402R (imenovan tudi LMW8-DR), ki kodira beljakovino CD2v, ki je homologna glodavskemu, cloveskemu in prasicjemu T-celicnemu adhezivnemu receptorju. Pokazalo se je, da je CD2v neposredno vpletena v proces hemadsorpcije in je izražena v celicah, okuženih z ASFV kot glikoprotein z molekulsko maso okrog 105-110 kDa. V prisotnosti zaviralca glikozilacije tunicamicina je njegova molekulska masa približno 42 kDa. V celicah, okuženih z ASFV, oznacenih s 3 H-glukozaminom ali 14 C-natrijevim acetatom, je bil s testom radioimunoprecipitacije ugotovljen virusno specificni osrednji glikoprotein z molekulsko maso 110-140 kDa (gp 110-140). Z uporabo referencnih sevov ASFV, ki pripadajo seroimunotipom I-IV, in ustreznim antiserumom, ki so bili aktivni pri preizkusu zaviranja hemadsorpcije (HADIA), smo ugotovili, da gp 110-140 doloca specificnost serotipa. Genotipizacija na osnovi genskega lokusa, ki kodira CD2v in C-tip lektinske beljakovine, je pokazala soizražanje s skupino izolatov in sevov ASFV na podlagi njihovih seroimunotipov. Imunizacija prasicev z gp 110-140 v liposomih ali z rekombinantnim hemaglutininom (CD2v) je zascitila od 67 do 100 odstotkov živali pred smrtjo zaradi njihove naknadne okužbe z virulentnimi sevi ASFV. Na podlagi fizikalno-kemicnih in bioloskih znacilnosti beljakovin gp 110-140 in CD2v menimo, da gre za isti virusni glikoprotein, ki je kljucnega pomena za vzpodbuditev imunoloske zascite pred ASF. Kljucne besede: ASFV; seroimunotipi; serotip; glikoproteini; gp 110-140; CD2v; zascita

SEROTYPE-SPECIFIC AND HAEMADSORPTION PROTEIN OF THE AFRICAN SWINE FEVER VIRUS