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Conservation of black orchids (Coelogyne pandurata Lindl.) is a high priority because the natural population is decreasing significantly. A typical method for orchid conservation is farming. The aims of this research were to examine the effects of adding benzyladenine (BA) to medium on the total number of buds, shoot initiation, and the number and length of roots. Our research method was based on a completely randomized design, in which various concentrations of BA (0, 0.5, 1, 1.5 and 2 ppm) were added to Vacin and Went medium. The BA concentration significantly affected the growth of black orchid plantlets. The most prominent effect was seen at 0.5 and 1 ppm/L BA; however, increasing the concentration over 1 ppm/L inhibited bud growth and the number and length of roots. Our data suggest that optimization of the BA concentration added to medium leads to accelerated growth of black orchid plantlets. In vitro propagation with the addition of BA to Vacin and Went media can be applied for black orchid conservation.

Effect of Benzyl Adenin on the Growth of Black Orchid ( Coelogyne pandurata lindl.) Planlets in in vitro Culture