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espanolMuestras de hongos fueron recolectadas en las estribaciones occidentales y orientales de Ecuador en las provincias de Pichincha, Sucumbios, Orellana y Santo Domingo de los Tsachilas. Los hongos fueron asepticamente aislados y multiplicados en Agar Papa Dextrosa (PDA) o Agar Malta (MA). Se observo su crecimiento y luego fueron identificados molecularmente en base a la secuencia de la region ITS1 e ITS2 del ADN ribosomal. Las secuencias obtenidas fueron comparadas con otras similares en Genbank para su analisis filogenetico e identificacion. Las cepas aisladas e identificadas fueron sembradas en Carboximetilcelulosa (CMC) para evaluar sus capacidades enzimaticas de degradacion celulosica. Diametros (en mm) de los halos de hidrolisis fueron continuamente monitoreados durante 7 y 14 dias de crecimiento, observandose halos mas grandes en las cepas H23.2 y H24 del genero Pestalotiopsis y la de L4 del genero Neonothopanus con medias significativas. Mayor actividad extracelulasica se registro en los sobrenadantes extraidos de los generos Neonothopanus, Pestalotiopsis y Gymnopilus a los 14 dias de cultivo en el intervalo de 24 h. Esta actividad enzimatica en algunos casos no guardo una relacion proporcional con la concentracion proteica en μg/ml de los sobrenadantes extraidos de los diferentes cultivos. EnglishFungal samples were collected in the Western and Eastern Andean foothills of Ecuador in the provinces of Pichincha, Sucumbios, Orellana and Santo Domingo de los Tsachilas. The fungi were aseptically isolated and increased on Potato Dextrose Agar (PDA) or Malt Agar (MA). Their growth characteristics were recorded, and molecularly identified on the basis of ribosomal RNA internal transcribed spacer (ITS) region. The sequences were compared with other similar sequences on GenBank for phylogenetic analysis and identification. The isolated and identified strains were plated on Carboxymethylcellulose (CMC) to evaluate their enzymatic capabilities to degrade cellulose. Diameters (in mm) of the halos of hydrolysis were continuously monitored during 7-14 days of growth. The halos in strains H23.2 and H24 of the genus Pestalotiopsis and of L4 of the genus Neonothopanus had halo diameters significantly bigger than most of the other fungal samples tested. Increased extracellulolytic activity was recorded in the supernatants extracted from the genera Pestalotiopsis, Neonothopanus, and Gymnopilus after 14 days of culture in the 24-hour interval. This enzyme activity, in some cases, was not proportional to the protein concentration in μg/ml of the supernatants extracted from the different cultures

Identificación y evaluación de algunos hongos con actividad celulásica aislados en Ecuador