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Experimental Procedure: Sixty C57BL/6 mice were divided into six groups as follow: Group 1, E7d-ALUM-GEL; Group 2, E7d-ALUM; Group 3, E7d-MONTANIDE ISA 206GEL; Group 4, E7d-MONTANIDE ISA 206; Group 5 and Group 6, GEL ALONE and PBS . The oral feeding of Aloe Vera gel with 0.05% polysaccharide was done for a period of 30 days. Mice were immunized with HPV16 E7d vaccine three times on day 0, 14 and 28th. Then tumor was implanted subcoutaneosly and 2 weeks later, the serum samples of the mice were collected to evaluate IL-4 and IFN-γ cytokines and specific total IgG, IgG1 and IgG2a antibodies levels by ELISA.

Aloe Vera Extracted Polysaccharides Shift the Immune Responses of Tumor Bearing Mice Toward Th1 Pattern: Animal Study