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This study was aimed at production of cellulase, amylase, tannase and ethanol by co-culturing of Bacillus megaterium and Saccharomyces cerevisiae using fruit waste as the sole substrate in submerge fermentation. Maximum amylase production (1.208 ± 0.14 IU/mL) was observed in fresh melon peel substrate after 48 h of fermentation. Maximum filter paper activity (0.645 ± 0.02 IU/mL) and tannase production (0.25 ± 0.01 IU/mL) was obtained from rotten peach after 24 h of fermentation. Sweet lemon peels yielded highest carboxymethyl cellulase production (0.435± 0.02 IU/mL) after 24 h of fermentation. Maximum reducing sugars (2.537± 0.07 mg/mL) and total sugars (17.703 ± 0.13 mg/mL) liberation was observed from sweet lemon peels after 24h of fermentation. Maximum ethanol production (0.56 ± 0.03 mg/ mL) was also found from sweet lemon peel after 72h of fermentation. Findings of this study showed that sweet lemon peels had potential for production of biologically active compounds via microbial fermentation.

Utilization of fruit wastes for enzyme production in submerged fermentation