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ANTIMICROBIAL ACTIVITIES AND PHYTOCHEMICAL INVESTIGATION OF FERN, LYGODIUM FLEXUOSUM (LINN) SW. Jeeshna, M.V. Department of Botany, Sree Narayana College, Kannur, Kerala-670 009. E-mail: mvjeeshna@gmail.com The present study revealed the presence of many medicinally active constituent in Lygodium flexuosum, suggesting that this species have potential to synthesize useful secondary metabolites. In this studies two different types (vegetative material and sporophyll bearing type) of this species showed the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, phenols and glycosides. In which vegetative material showed the presence of all secondary metabolites. The low polar solvent extracts such as petroleum ether and acetone showed minimum presence of secondary metabolites. The antibacterial studies revealed that methanol extracts of sporophyll type exhibited significant activity (8.5 mm) against the bacterium, Klebsiella pneumonia. The next antibacterial activity showed by chloroform extracts of sporophyll type against the same bacteria (8.2 mm). In antifungal studies, methanol extracts of vegetative material has the highest inhibitory activity (19.3 mm) against the fungus Cladosporium sp. Next higher fungal activity was showed by same extract of sporophyll type against the fungus Rhizopus sp. (16.08 mm). The study confirms the antimicrobial potential of Lygodium flexuosum extracted using various solvents.