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The plant diversity of Bukit Timah Nature Reserve (BTNR) is relatively well studied due to concerted effort over several decades, particularly as part of the worldwide system of ecological plots set up by the Center for Tropical Forest Science (CTFS), now called the Forest Global Earth Observatory. Publications arising from previous works have set baseline data for the species diversity, suggested that the forest resilience was greater than would be expected in such a small forest fragment, but that there was low recruitment of primary forest tree species into the secondary forest. In order to assess the overall vascular plant diversity, and to compare the diversity of the various forest types within BTNR to each other, 52 plots were set up, each 20 × 5 m, along nine different transects that covered the full range of topography and forest types, primary, old secondary and maturing secondary forests, within the reserve. The vascular plant diversity within each plot was recorded. In total, 1250 species in 148 families were recorded, including an additional 167 species newly listed for BTNR. The primary forest had the highest number of species not found in the other forest types. It nevertheless had a very large overlap with species in the old secondary forest but not with the maturing secondary forest.

The plant diversity in Bukit Timah Nature Reserve, Singapore