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To detect S-phase cells in the decalcified paraffin-embedded temporomandibular joint (TMJ) tissues of the rat, bromodeoxyuridine (BrdUrd) immunohistochemistry was performed. Percardiac perfusion of 10% EDTA-2Na Tris buffer accelerated the decalcification. The use of the monoclonal antibody to BrdUrd enabled the visualization of the distribution of S-phase cells. This method is practical in studying cell kinetics and the specific proliferation mode of the TMJ.

Application of Bromodeoxyuridine Immunohistochemistry to the Rat Temporomandibular Joint