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In a single ultrathin section, only some features of the cell organelles in a cell can be observed. However, several serial sections can give three-dimensional findings on the cell organelles. For example, I have observed the process of exocytosis of secretory granules in the prolactin (PRL) cells in mouse adenohypophysis by the observation of several serial ultrathin sections (Sasaki, 1974). By the sectioning of 60 semi-ultrathin serial sections 0.5 pm thick on an ultramicrotome and the making of models of the three-dimensional structure of the PRL cells, a clear sexdifference was observed in an external view of the PRL cells (Sasaki and Sano, 1977). It was difficult to cut thick serial sections uniformly more than 5 pm thick for light microscopy from Epon-embedded blocks on an ultramicrotome. The need for such thick sections is often keenly felt. West (1972) reported a method for cutting thick sections for light microscopy from epoxy blocks. However, the Epon is too hard for such objects to allow thick sections in good condition. I have also tried to devise such method (Sasaki and Sano, 1986). With the method reported here, Epon-embedded tissues can be more easily and uniformly sectioned into thick serial sections for light microscopy than with West's method. Pituitary glands from adult female rats and mice were cut in the midsagittal plane. The right and left halves of each pituitary gland in rats were diced into four (the tissue was then about 2 mm in its maximum length) and 12 pieces, respectively, both sizes of which could be fixed satisfactorily for electron microscopy. The right and left hemipituitaries in mice were also diced into 2 and 6 pieces, respectively. These tissue blocks were fixed in formaldehyde-glutaraldehyde fixative (Ito and Karnovsky, 1968) and postfixed in 107o

An Improved Method for Cutting of Thick Serial Sections for Light Microscopy from Epon-embedded Tissues