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Mucous connective tissue in adult animals is found, as is well known, in the dental pulp where the extracellular substance is abundant and the tissue has a gelatinous consistency. To date, electron microscope observations on the dental pulp are numerous. A very and H a n (1961) investigated on the formation of collagen fibrils in the dental pulp of a newborn hamster, and H a n, A very and Hale (1965) studied on the differentiation of the fibroblasts in the incisor pulp of the guinea pig. Tooth germ was also investigated electron microscopically by Avery and Watson (1954), Lenz (1959), Nakamura et al. (1959), Reith (1960, 1961, 1962) and Pannese (1962). Very recently R e i t h's work dealing with the fibrogenesis in developing molar teeth of rats appeared (1968). Aside from these observations, however, little is known about the formation and differentiation of the extracellular substance of the dental pulp. In view of this, the present study is concerned with the differentiation of the fibroblasts and the histochemical characterization of the matrix substance of the developing pulp, especially in the field of fine structure.

Electron Microscope Observations of the Developing Rat Dental Pulp with Special Reference to the Formation of the Extracellular Substance