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Special Reagents : 0.5 per cent aqueous solution ofbenzidine 95 cc. Hydrogen peroxide (3 per cent)••-•10 gtt. (Sahli) 1 per cent aqueous solution of nickel-ammonium sulfate (NAS) 5 cc. 0.5 Gm. of benzidine (Benzidin puriss, E. Merck) is dissolved in 100 cc. of distilled water, and it is necessary to rub benzidine in a mortar unless fine powder. The preparation is filtered and ten drops (S a hl i) of hydrogen peroxide are added, finally to its 95 cc. are added 5 cc. of 1 per cent aqueous solution of Nickel-Ammonium Sulfate (NAS is the capitals of this chemical). The NAS-Benzidine solution is thus prepared, which should have a pH of 5.8-6.0 approximately. Namely the mixture is necessary which includes 95 parts of benzidine solution, 5 parts of NAS-solution, and 10 drops of hydrogen peroxide per 100 cc. of the mixture. If this NAS-Benzidine solution is kept in the dark when not in use, it will keep half a year without significant loss of potency.

NAS-Benzidine Reaction of Blood Cells-New Simple Peroxidase Staining Method Using Nickel-Ammonium Sulfate