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The light distribution in vivo during Photodynamic Therapy is dependent on a pre-administered photosensitizer in the target tissue. To discuss the light absorption of photosensitizer(ME2906) ,the light distribution in noinial muscle at 664 nm was measured with optical detector fiber. From this distribution , the absorption coefficient of ME2906 in tissue was calculated. As an optical source, a semiconductor laser is prepared. The incident laser beam diameter at the tissue surface is 10 mm, and the power density was 100 mW/cm2. In proportion as the concentration of ME2906 increases, absorption coefficient of ME2906 increases in the range from 0.06 to 0.16 mm-1. This coefficients are related to ME2906 concentrations in the plasma and tissue. Therefore, ME2906 concentrations of plasma were examined by high-performance liquid chromatography (HPLC).

The measurement of the absorption coefficient of ME2906 in tissue using a semiconductor laser