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To observe the microcirculation in a rather deep portionin the parenchymal organ, we have designed a new in vivo microscope by dual slit lazer beams illumination, in which the fluorescent lighte mitted from the tracer by these beams in the crossing area alone can be picked up. This microscope was appliedto observe the tomographic image of microvasculature and to analyze the macromolecular permeability of single capillary in the anesthetized rabbit tenuisimus muscle. The obtained images make a high vertical zone selectivity of tomographic image from muscle surface to 200 ,ƒÊm in depth. Furthermore, the quick macromolecular leakage can be observed in the single venular capillary region. It was concluded that present method is useful to quantify the microvasculature and capillary permeabilityunder in vivo study.

Measurements of the microcirculation in a rather deep portion in the parenchymal organ by using laser vital-microscope