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In photodynamic therapy, the development of effective photosensitizers, which absorb the light with long penetration depth for tissue, has been required. In this study, methylen blue, which has the relatively long absorption wavelength at 665nm, was applied for photodynamic therapy. Methylen blue was easily incorporated into HeLa cells. BY the laser irradiation of HeLa cells containing methylen blue, the cells were strongly damaged. From the electron microscopic observations methylen blue, seems to be suitable in photodynamic therapy in clinic.

PHOTODYNAMIC THERAPY WITH METHYLEN BLUE AS A SENSITIZER