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The medicinal plant water spinach is applicable for many therapeutic activities like anti cancer, anti oxidant, etc. the plant is used for purification of metals and waters of different categories. The benefit of hydroalcoholic extract in this study is non toxic, preservative and high yield of extract from the herbal powder. Thin layer chromatographic analysis is one of the best analytical methods for the separation and identification of phytoconstituents from the crude extract. The spots separated on the stationary phase could be detected by using the non destructive fluorescence method. The detection will be done at shorter and longer wavelength in UV cabinet. Aim and Objective: The aim was to separate and identify the various active metabolites present in the crude extract of the herb of interest using Rf value by thin layer chromatographic analysis. Method: The thin layer chromatographic parameters were set by trial and error method. Ethyl acetate: Methanol: Water (10.0:1.35:1.0 v/v/v) for alkaloids, Ethyl acetate: Pyridine: Water (5.0:1.0:4.0 v/v/v) for glycosides, Toluene: Acetone: Formic acid (4.5:4.5:1.0 v/v/v) for tannins and Toluene: Ethyl acetate (9.3:0.7 v/v) for triterpenoids were the mobile phases for the individual constituents. The constituents were identified at 365nm. Results and Discussion: Nearly 24 various constituents of alkaloids, glycosides, flavonoids, triterpenoids and tannins were separated using respective mobile phases through Rf value. Conclusion: The mentioned TLC profile for the plant of interest will hopefully help the researchers in herbal field to develop more herbal medicines requiring the particular constituent or its extract.

Fluorescence Detection of Phytoconstituents in Hydroalcoholic Extract of Ipomoea aquatica Forssk. by Thin Layer Chromatographic Analysis