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Efficient conditions for plant regeneration using adventitious buds differentiated from Atropa belladonna L. hairy roots under shaking culture were investigated. In the combination of relative strength of MS medium and various sucrose concentrations in liquid medium (hormone free), shoot formation was promoted at 5 and 10 g L-1 sucrose. Root formation was stimulated at 30 g L-1 sucrose, while shoot formation was inhibited. The total fresh weight increased with increasing the strength of MS medium but decreased strongly at doublestrength MS medium containing 90 g L-1 sucrose. Multiple shoots with roots were obtained most efficiently from adventitious buds for one month of culture under normal-strength MS medium with 30 g L-1 sucrose. These results suggest the possibility of mass propagation of shoots and application to the production of young plants from propagated shoots.

Efficient Conditions for Plant Regeneration Using Adventitious Buds Differentiated from Atropa Belladonna L. Hairy Roots Under Shaking Culture