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To evaluate the influence of a fluoride mouthwash containing nanohydroxyapatite on the dentin bonding interface microhardness of direct resin composite restorations in situ. Methodology: Forty human premolars were sectioned and restored with a three-step adhesive system or a self-etch adhesive system and a conventional resin-based composite. After the initial microhardness measurements on the samples’ dentin interface, the restored samples were fixed in a crylic palate a ppliances for a n in situ experiment. Ten volunteers participated in a randomized double-blinded crossover study using the palate appliances and a placebo fluoride mouthwash for one week, one-week wash-out, and one-week fluoride mouthwash containing nanohydroxyapatite. The final microhardness measurements were evaluated, and data were analyzed by ANOVA and Tukey’s test submitted for multiple comparisons (α = 0.05; 0.8 power). Results: There were statistically significant differences be tween the mouthwash treatments with both adhesive systems tested (p < 0.001). The nanohydroxyapatite used in the mouthwash was an influencing actor on the dentin interface microhardness of resin composite restorations. Conclusion: The fluoride m outhwash c ontaining t he n anohydroxyapatite w as a ble t o increase the dentin microhardness of the direct resin composite restorations regardless of the adhesive system used.

stomatology edu journal

Influence of fluoride mouthwash containing nanohydroxyapatite on the dentin interface of direct restorations: a randomized controlled crossover in situ study