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We isolated alkalophilic bacterium, Agromyces sp. KY5R, which degrades 6-aminohexanoate (Ahx)-oligomer (by-product of nylon-6 manufacture, designated as nylon-oligomer), from activated sludge of sewage disposal plant. The strain KY5R exhibited good growth on LBplate containing insoluble Ahx-oligomer (pH10), and produced clear zone by hydrolysis of the oligomers. 6-Aminohexanoate-oligomer hydrolase from KY5R (A-EIII) had optimum pH at 7.5-8.0. This value was 0.5 point higher than the optimum pH of P-EIII (EIII encoded on plasmid pOAD2 in Arthrobacter sp. KI72). In addition, thermostability test revealed that the A-EIII was stable up to 60 °C, while the P-EIII was stable up to 50 °C under the same condition. Cloning and sequencing analysis demonstrated that the amino acid sequence of the two EIII enzymes differs only at 5 positions among 355 amino acid residues. These results suggest that at least one of the fi ve alterations affects the differences in the enzyme characteristics.

Isolation of alkalophilic nylon-oligomer-degrading bacterium: Cloning and sequencing analysis of 6-aminohexanoate-oligomer hydrolase