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This study aimed to assess the effect of polyphenols in turkey berry fruits on level of antioxidant enzyme (erythrocyte superoxide dismutation [SOD]) and oxidative stress marker (serum 8-isoprostane) in obese adult women. The study was quasi experimental trial with cross-over design. Eight obese women subjects in Benteng Village-Ciampea, Bogor District, West Java were randomly assigned to a control group (n=4), and a turkey berry group (n=4) for 2 x 1 week daily treatment with a one week wash-out period between treatment periods. Turkey berry group consumed home diet and ±160 g of cooked turkey berry (as a side dish or snack) while the control group consumed only home diet. A food record consumption data was collected twice each week. Blood samples were obtained in the morning at day 1, 8, 15, and 22 of interventions and analyzed for the level of erythrocyte SOD and serum 8-isoprostane. The results showed that the different levels of erythrocyte SOD between post and pre intervention on turkey berry groups was not significantly different compared to the control group (p=0.607). Whereas, the different level of serum 8-isoprostane between post and pre intervention on turkey berry group in which decreasing was significantly different from the level on control group which was increasing (p<0.05). It seems that daily consumption of cooked turkey berry fruits can improve oxidative stress condition. Keywords: enzyme antioxidant, oxidative stress, polyphenol ABSTRAK Tujuan penelitian ini adalah untuk mengkaji pengaruh polifenol dalam buah takokak terhadap kadar enzim antioksidan (superoksida dismutase [SOD] eritrosit) dan penanda stres oksidatif (8-isoprostan serum) pada wanita dewasa gemuk. Penelitian ini merupakan studi kuasi eksperimental dengan desain cross-over, sebanyak 8 subjek wanita gemuk di Desa Benteng-Ciampea, Kabupaten Bogor, Jawa Barat secara acak dibagi rata pada kelompok kontrol (n=4) dan kelompok takokak (n=4) selama 2 x 1 minggu perlakuan dengan 1 minggu periode wash-out antar periode perlakuan. Kelompok takokak mengonsumsi home diet dan ±160 g takokak masak (sebagai lauk atau camilan), sedangkan kelompok kontrol hanya mengonsumsi home diet. Data konsumsi pangan dikumpulkan dengan teknik food record 1x24 jam sebanyak dua kali setiap minggu. Sampel darah diambil pagi hari pada hari ke-1, ke-8, ke-15, dan ke-22 intervensi untuk dianalisis kadar superoksida dismutase (SOD) eritrosit dan 8-isoprostan serum. Hasil penelitian menunjukkan bahwa perbedaan kadar SOD eritrosit antara setelah dan sebelum intervensi pada kelompok takokak tidak berbeda signifikan dibandingkan dengan kelompok kontrol (p=0,607). Perbedaan kadar 8-isoprostan serum antara setelah dan sebelum intervensi pada kelompok takokak yang mengalami penurunan berbeda signifikan dengan kelompok kontrol yang mengalami peningkatan (p<0,05). Hasil ini memperlihatkan bahwa konsumsi buah takokak masak sehari-hari dapat memperbaiki kondisi stres oksidatif. Kata kunci: antioksidan enzim, polifenol, stres oksidatif

EFEK INTERVENSI BUAH TAKOKAK (Solanum torvum Swartz) TERHADAP KADAR SUPEROKSIDA DISMUTASE ERITROSIT DAN 8-ISOPROSTAN SERUM PADA WANITA DEWASA GEMUK