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Genomic DNA has become a very important source for multiple purposes in research projects. Therefore, the analysis of the quality of the extraction of DNA using different specimen collection methods; are part of every materials and method section in current papers (17). The objective of this study was to evaluate which protocol extracts the highest yield with better quality of DNA from human buccal swabs. There are two different ways to harvest DNA from buccal swabs: single or multi-step procedure. Our study compared the quality of the DNA extracted using these two different methods of DNA extraction; though the determination spectroscopic measurements at 280, 260, and 230 nm wavelengths and the suitability for application in genotyping assays.

COMPARISON OF THE QUALITY OF DNA EXTRACTED FROM BUCCAL SWABS USING SINGLE STEP AND MULTI-STEP PROCEDURES