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The stress of pesticides is a very common factor today in human and animal health. Fishes can serve as bio indicators of environmental pollution, since they are directly exposed to chemicals resulting from agricultural production via surface run off or directly through the food chain of the ecosystem. Haematological parameters, protein levels etc , are considered as the index of the total body and therefore they are important in diagnosing structural and functional strategy of fish. Two different stages of fishes (juveniles and adults) were chosen for experimenting and a narrow range of pesticide chlorpyrifos was used in juvenile fishes( ranging from 10ppm to 30ppm) and in adults two different concentrations(both narrow and broad) range of pesticide were used The broad range concentration is 1000ppm or 1ml. And in narrow range in adults, three different concentrations were used (10ppm,20ppm, and 30ppm). Various biochemical and histological parameters of the pesticide treated fishes were tested and compared with that of the control. This proved the toxic effect of the pesticide in the aquatic habitat. INTRODUCTION Water pollution problems related to agrochemicals have been taken into consideration and it has been found that use of agrochemicals in the crop fields is liable to change the abiotic and biotic characters of the aquatic media leading hazards to the aquatic flora and fauna. Generally, the biochemical parameters serve as indicators for monitoring the pathological status of the treated fish. These variations were tissue specific and species specific, but it can be used as an indicator for pesticide pollution. Such differential behaviour with regard to tissue damage will be markers to characterize the particular pollutant and its potential toxicity to fish. Fishes are rich in proteins, lipids, vitamins etc., and form valuable food for the people throughout the world and especially for the people suffering from malnutrition. Fishes are aquatic and poikilothermic animals and perform best under narrow range of optimum environmental conditions. Pesticides are used to control pests of food crop, livestock and human health. Due to the use of pesticides, water bodies like ponds, lakes and low lying water filled areas are continuously polluted. The pesticides are used in the agricultural fields for the eradication of the insects, IMPACT OF PESTICIDE CHLORPYRIFOS ON THE FRESH WATER FISH, OREOCHROMIS SPP. SDRP Journal of Cellular and Molecular Physiology