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One of the major obstacles in DNA processing and analysis is the presence of inhibitors which can be co-extracted with the genetic material or are an inherent part of the sample and can interfere with downstream processing. Analysts continue to struggle with the processing and interpretation of such samples. While major forensic kit manufacturers have made great strides in improving the resistance of PCR kits to inhibition, challenges remain when it comes to identifying, and overcoming the effects of inhibitors in biological samples. The goal of this project is to assess the relationship between the quantification results obtained with Quantifiler® Duo and its downstream effect on amplification with the Identifiler® Plus STR kit by spiking samples with inhibitor concentrations that may result from carryover following sample extraction. While the Quantification kit is effective at detecting inhibition for certain compounds at the concentration levels examined, substances such as humic acid and phenol may not show strong inhibitory affects during real-time PCR assays yet can negatively influence downstream STR typing. Therefore analysts should practice discretion when using IPCs for inhibition detection as real-time PCR systems can differ in response depending on the type of PCR inhibitor present.

The Effect of Sample and Sample Matrix on DNA Processing: Mechanisms for the Detection and Management of Inhibition in Forensic Samples