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OF THE THESIS CHARACTERIZATION AND INTERACTIONS OF MERCURY, DISSOLVED ORGANIC MATTER AND ORGANIC SULFUR IN SURFACE WATERS OF THE FLORIDA EVERGLADES by Maria Asuncion Calero Sheils Florida International University, 2003 Miami, Florida Professor Yong Cai, Co-Major Professor Professor Rudolf Jaffe, Co-Major Professor Two sampling stations in the Florida Everglades were selected based on the results from US Environmental Protection Agency and Florida International University Mercury Project. In both sites, most of the methylmercury is associated with the truly dissolved organic matter fraction (<3KDa), where total sulfur levels are also higher. Characterization of size fractionated dissolved organic matter by synchronous fluorescence, UV spectrometry and 13C-NMR was accomplished, revealing a decrease in the aromatic to aliphatic ratio in the DOM functionalities as the molecular weight of the fractions decrease. The determination of organic thiols at trace levels has been achieved in both particulate and dissolved phases by sensitive detection with fluorescence after a reverse-phase liquid chromatographic separation. The majority of the thiols are present in the dissolved fraction in surface waters of the Everglades. Three uncharacterized thiols were found for the first time in surface waters of the Florida Everglades.

Characterization and interactions of mercury, dissolved organic matter and organic sulfur in surface waters of the Florida Everglades