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From gut content (ileum and cecum) of eight turkeys, 73 days old, was isolated, phenotypic identified and preserved, nine strains from Lactobacillus genus (L. acidophilus IBNA 11, L. acidophilus biotype 3 IBNA 12, L. fermentum biotype 1, IBNA 13-18 and L. salivarius IBNA 19) and one strain of Enterococcus faecium (IBNA 10). The parallel identification of strains was made by apiweb API50CHL V.5.1, BioMerieux (France) software, API20STREP, and ABIS online software. Quantitative level of Lactobacillus spp. strains (10 – 10 CFU/g intestinal content) and Enterococcus faecium strain (10CFU/g intestinal content) in ecological niche was determined. The viability of Lactobacillus spp. strains preserved at 4C (from 45 to 90 days) and at room temperature (under 90 days), and Enterococcus faecium at 4C (more than 11 months) was tested.

Lactobacillus spp. and Enterococcus faecium strains isolation, identification, preservation and quantitative determinations from turkey gut content