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Clerodendrum thomsoniae commonly known as bleeding heart vine or bag flower. In this study, in-vitro callus regeneration of C. thomsoniae through nodal culture has been tried. Murashige and Skoog’s medium (MS) with BAP was found suitable for shoot and root development. To detect clonal fidelity in C. thomsoniae, RAPD and ISSR markers were used. Ten RAPD decamers produced 65 amplicons, while ten ISSR primers generated 75 bands in both in-vitro plantlets and mother plants. The amplified products of parent plants and the regenerated plants were found to be monomorphic in RAPD and ISSR analyses. A number of compounds with potential therapeutic and biological activity had been detected with the help of GC-MS analysis. Among this the compounds namely 3-Hydroxybutyric acid, Azelaic acid, Linoleic acid, Oleic acid, Squalene, Stigmasterol are the important compounds mainly responsible for anti-cancer, anti-inflammatory, antiasthma, antimicrobial, antistress activity and so on. Taking into account of all the GC-MS and in-silico molecular docking data, it can be concluded that some of these compounds may be potential to future drug industry.

research in plant biology

Micropropagation, genetic fidelity assessment and phytochemical studies of Clerodendrum thomsoniae Balf. f. with special reference to its anti-stress properties