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Plants have been used invariably by humans since ages for food, medicines and various other day to day needs. However, increasing population and incessant human needs have put enormous pressure on these bio-resources and lead to their unprecedented depletion from nature. To address this problem, the tissue culture techniques have come to the rescue of depleting plants [1]. Plant tissue culture has been extensively used to boost the large scale micro-propagation of threatened medicinal plants [2]. This not only helps in large scale production of true-to-type plantlets but also helps to conserve rare and threatened plants.

Callus induction and axillary shoot formation in Asparagus racemosus Willd.