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The effect of galanin was studied on the release of growth hormone (GH) from perifused medial basal hypothalami (MBH) and/or adenohypophyses of steers in a sequential double chamber perifusion system. Galanin stimulated significant GH release at the doses of 10-7, 10-8 (P<0.01) and 10-9M (P<0.05) in perifusion groups containing pituitary fragments alone compared with control perifusion group. The elevation induced by galanin in perifusion groups containing pituitary fragment alone tended to be in a dose dependent manner. In contrast to these results, galanin stimulated significant GH release only at the dose of 10-7M in perifusion groups containing both the hypothalamus and pituitary fragments in tandem (P<0.01). The present results suggest that galanin stimulates GH release by acting directly on the adenohypophysis in cattle. Anim. Sci. Technol. (Jpn.) 64 (6) : 553-560, 1993

Effect of Galanin on the Release of Growth Hormone in Perifused Bovine Pituitary and Hypothalamus