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A growth-stimulating substance for specific lactic cultures such as Lactobacillus casei and Lactobacillus acidophilus was isolated from a precipitate fraction of soybean whey which was followed by ethanol fractionation , and some properties of the substance were investigated. Crude preparation fractionated with ethanol was passed through sulphopropyl-Sephadex C-25 and was resolved into at least three active growth stimulatory fractions of Lactobacillus casei subsp. alactosus 34143 used as the test organism of microbiological assays . One of the fractions, which showed the highest activity , was homogenous and ninhydrin positive. From the results of UV absorption spectrum and amino acid analysis, the purified growth-stimulating substance was considered to be peptide and its molecular weight 1,150 daltons . Jpn. J. Zootech. Sci., 58 (9): 754-763 , 1987

Isolation from Soy Milk of a Growth-stimulating Substance for Lactic Acid Bacteria