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The role of volatile fatty acids in postnatal development of rumen, including the growth of the ruminal papillae or the increase of epitherial cell mass, has been extensively studied in vivo. TAMATE et al.1) demonstrated that the mitotic index of the epithelial cells of the rumen in sheep fed with barley increased and their deletion decreased. Also, it was reported that the mitotic index of the epithelial cells of the sheep rumen administrated intraruminally butyrate, propionate and acetate2,3,4), increased significantly than that of untreated rumen (standard conditions). However, there is no evidence to postulate whether volatile fatty acids increase the mitotic index of the epithelial cells directly or indirectly5). This is due to the fact that the in vitro study, using the epithelial cell culture, has not properly developed. In this experiment, in connection with a study on the role of volatile fatty acids in the mitotic effect of epithelial cells, we investigated the cell culture technique, using the rumen mucosa of cattle. The present paper describes an approach, allowing rapidly cell culture from the rumen mucosa of cattle.

A Simple Primary Culture of Epithelial Cells from Rumen Mucosa of Cattle